SIMULTANEOUS ESTIMATION OF BERBERINE HYDROCHLORIDE AND PLUMBAGIN IN MARKETED POLYHERBAL FORMULATION BY RP-HPLC
A reverse phase high performance liquid chromatographic method has been developed for the estimation of Berberine hydrochloride and Plumbagin in polyherbal formulations. The method is simple, precise and accurate. The method is based on the separation eluted using a mobile phase mixture of methanol and 0.1% ammonium acetate solution in a ratio of 60:40 %v/v at a flow rate of 1.0ml/min. The detection was made at 280 nm. The retention times were 3.01 for Berberine hydrochloride and 8.21min for Plumbagin. Calibration curve was linear over the concentration range of 50-150μg/ml for Berberine hydrochloride and 20-80 μg/ml for Plumbagin. The accuracy of the method was assessed by recovery studies and was found to be for 100.04% for Berberine hydrochloride and 100.06% for Plumbagin. The developed method was validated as per the ICH guidelines parameters like Linearity, precision, accuracy, robustness, LOD and LOQ. The results were validated as per ICH Q2 R1 guideline and were satisfactory.
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