Method Development and Validation for Determination of Ramipril and Metoprolol Succinate by Zero Order Derivative in bulk and Marketed Formulation

Sitaram Atmaji Naik


The proposed method involves Zero order derivative spectroscopy method. A novel, simple and rapid UV Spectrophotometric determination method for Simultaneous estimation of Ramipril (RAM) and Metoprolol Succinate (METO) was successfully developed and validated in bulk and pharmaceutical formulation. In this current work simultaneous estimation of RAM and METO in Methanol was performed by using UV spectroscopy. The zero order derivative spectroscopy revealed that RAM had a λmax of 205nm in Methanol and METO had λmax of 275nm respectively. The linearity was found in the range of 3-30μg/ml for RAM and 20-200μg/ml for METO. The R2 value was found to be 0.9994 and slope y=0.0365 for RAM and R2 =0.9986 and slope y=0.0054 for METO.


Ramipril, Metoprolol Succinate, Zero order derivative method,


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